Pathology of experimental SARS coronavirus infection in cats and ferrets.
Identifieur interne : 003063 ( Main/Exploration ); précédent : 003062; suivant : 003064Pathology of experimental SARS coronavirus infection in cats and ferrets.
Auteurs : J M A. Van Den Brand [Pays-Bas] ; B L Haagmans ; L. Leijten ; D. Van Riel ; B E E. Martina ; A D M E. Osterhaus ; T. KuikenSource :
- Veterinary pathology [ 0300-9858 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Antigènes viraux (métabolisme), Chats (virologie), Furets (virologie), Immunohistochimie, Infections de l'appareil respiratoire (anatomopathologie), Infections de l'appareil respiratoire (enzymologie), Infections de l'appareil respiratoire (virologie), Modèles animaux de maladie humaine, Organismes exempts d'organismes pathogènes spécifiques, Peptidyl-Dipeptidase A (métabolisme), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (enzymologie), Syndrome respiratoire aigu sévère (virologie), Technique d'immunofluorescence, Virus du SRAS (croissance et développement).
- MESH :
- anatomopathologie : Infections de l'appareil respiratoire, Syndrome respiratoire aigu sévère.
- croissance et développement : Virus du SRAS.
- enzymologie : Infections de l'appareil respiratoire, Syndrome respiratoire aigu sévère.
- métabolisme : Antigènes viraux, Peptidyl-Dipeptidase A.
- virologie : Chats, Furets, Infections de l'appareil respiratoire, Syndrome respiratoire aigu sévère.
- Animaux, Immunohistochimie, Modèles animaux de maladie humaine, Organismes exempts d'organismes pathogènes spécifiques, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- Animals, Antigens, Viral (metabolism), Cats (virology), Disease Models, Animal, Ferrets (virology), Fluorescent Antibody Technique, Immunohistochemistry, Peptidyl-Dipeptidase A (metabolism), Respiratory Tract Infections (enzymology), Respiratory Tract Infections (pathology), Respiratory Tract Infections (virology), SARS Virus (growth & development), Severe Acute Respiratory Syndrome (enzymology), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology), Specific Pathogen-Free Organisms.
- MESH :
- chemical , metabolism : Antigens, Viral, Peptidyl-Dipeptidase A.
- enzymology : Respiratory Tract Infections, Severe Acute Respiratory Syndrome.
- growth & development : SARS Virus.
- pathology : Respiratory Tract Infections, Severe Acute Respiratory Syndrome.
- virology : Cats, Ferrets, Respiratory Tract Infections, Severe Acute Respiratory Syndrome.
- Animals, Disease Models, Animal, Fluorescent Antibody Technique, Immunohistochemistry, Specific Pathogen-Free Organisms.
Abstract
The pathology of severe acute respiratory syndrome-coronavirus (SARS-CoV) infection in cats and ferrets is poorly described, and the distribution of angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV, in the respiratory tracts of these species is unknown. We observed SARS-CoV antigen expression and lesions in the respiratory tracts of 4 cats and 4 ferrets at 4 days postinoculation and ACE2 expression in the respiratory tracts of 3 cats and 3 ferrets without infection. All infected cats and ferrets had diffuse alveolar damage associated with SARS-CoV antigen expression. A novel SARS-CoV-associated lesion was tracheo-bronchoadenitis in cats. SARS-CoV antigen expression occurred mainly in type I and II pneumocytes and serous cells of tracheo-bronchial submucosal glands of cats and in type II pneumocytes of ferrets. ACE2 expression occurred mainly in type I and II pneumocytes, tracheo-bronchial goblet cells, serous epithelial cells of tracheo-bronchial submucosal glands in cats, and type II pneumocytes and serous epithelial cells of tracheo-bronchial submucosal glands in ferrets. In conclusion, the pathology of SARS-CoV infection in cats and ferrets resembles that in humans except that syncytia and hyaline membranes were not observed. The identification of tracheo-bronchoadenitis in cats has potential implications for SARS pathogenesis and SARS-CoV excretion. Finally, these results show the importance of ACE2 expression for SARS-CoV infection in vivo: whereas ACE2 expression in type I and II pneumocytes in cats corresponded to SARS-CoV antigen expression in both cell types, expression of both ACE2 and SARS-CoV antigen in ferrets was limited mainly to type II pneumocytes.
DOI: 10.1354/vp.45-4-551
PubMed: 18587105
Affiliations:
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Leijten</name></author><author><name sortKey="Van Riel, D" sort="Van Riel, D" uniqKey="Van Riel D" first="D" last="Van Riel">D. Van Riel</name></author><author><name sortKey="Martina, B E E" sort="Martina, B E E" uniqKey="Martina B" first="B E E" last="Martina">B E E. Martina</name></author><author><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A D M E" last="Osterhaus">A D M E. Osterhaus</name></author><author><name sortKey="Kuiken, T" sort="Kuiken, T" uniqKey="Kuiken T" first="T" last="Kuiken">T. 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Van Den Brand</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Erasmus Medical Center, Dr. Molewaterplein 50, 3015 GE Rotterdam</wicri:regionArea><wicri:noRegion>3015 GE Rotterdam</wicri:noRegion></affiliation></author><author><name sortKey="Haagmans, B L" sort="Haagmans, B L" uniqKey="Haagmans B" first="B L" last="Haagmans">B L Haagmans</name></author><author><name sortKey="Leijten, L" sort="Leijten, L" uniqKey="Leijten L" first="L" last="Leijten">L. Leijten</name></author><author><name sortKey="Van Riel, D" sort="Van Riel, D" uniqKey="Van Riel D" first="D" last="Van Riel">D. Van Riel</name></author><author><name sortKey="Martina, B E E" sort="Martina, B E E" uniqKey="Martina B" first="B E E" last="Martina">B E E. Martina</name></author><author><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A D M E" last="Osterhaus">A D M E. Osterhaus</name></author><author><name sortKey="Kuiken, T" sort="Kuiken, T" uniqKey="Kuiken T" first="T" last="Kuiken">T. Kuiken</name></author></analytic><series><title level="j">Veterinary pathology</title><idno type="ISSN">0300-9858</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigens, Viral (metabolism)</term><term>Cats (virology)</term><term>Disease Models, Animal</term><term>Ferrets (virology)</term><term>Fluorescent Antibody Technique</term><term>Immunohistochemistry</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Respiratory Tract Infections (enzymology)</term><term>Respiratory Tract Infections (pathology)</term><term>Respiratory Tract Infections (virology)</term><term>SARS Virus (growth & development)</term><term>Severe Acute Respiratory Syndrome (enzymology)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Specific Pathogen-Free Organisms</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antigènes viraux (métabolisme)</term><term>Chats (virologie)</term><term>Furets (virologie)</term><term>Immunohistochimie</term><term>Infections de l'appareil respiratoire (anatomopathologie)</term><term>Infections de l'appareil respiratoire (enzymologie)</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Modèles animaux de maladie humaine</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (enzymologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique d'immunofluorescence</term><term>Virus du SRAS (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Respiratory Tract Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term><term>Peptidyl-Dipeptidase A</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Respiratory Tract Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chats</term><term>Furets</term><term>Infections de l'appareil respiratoire</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cats</term><term>Ferrets</term><term>Respiratory Tract Infections</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Fluorescent Antibody Technique</term><term>Immunohistochemistry</term><term>Specific Pathogen-Free Organisms</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Immunohistochimie</term><term>Modèles animaux de maladie humaine</term><term>Organismes exempts d'organismes pathogènes spécifiques</term><term>Technique d'immunofluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathology of severe acute respiratory syndrome-coronavirus (SARS-CoV) infection in cats and ferrets is poorly described, and the distribution of angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV, in the respiratory tracts of these species is unknown. We observed SARS-CoV antigen expression and lesions in the respiratory tracts of 4 cats and 4 ferrets at 4 days postinoculation and ACE2 expression in the respiratory tracts of 3 cats and 3 ferrets without infection. All infected cats and ferrets had diffuse alveolar damage associated with SARS-CoV antigen expression. A novel SARS-CoV-associated lesion was tracheo-bronchoadenitis in cats. SARS-CoV antigen expression occurred mainly in type I and II pneumocytes and serous cells of tracheo-bronchial submucosal glands of cats and in type II pneumocytes of ferrets. ACE2 expression occurred mainly in type I and II pneumocytes, tracheo-bronchial goblet cells, serous epithelial cells of tracheo-bronchial submucosal glands in cats, and type II pneumocytes and serous epithelial cells of tracheo-bronchial submucosal glands in ferrets. In conclusion, the pathology of SARS-CoV infection in cats and ferrets resembles that in humans except that syncytia and hyaline membranes were not observed. The identification of tracheo-bronchoadenitis in cats has potential implications for SARS pathogenesis and SARS-CoV excretion. Finally, these results show the importance of ACE2 expression for SARS-CoV infection in vivo: whereas ACE2 expression in type I and II pneumocytes in cats corresponded to SARS-CoV antigen expression in both cell types, expression of both ACE2 and SARS-CoV antigen in ferrets was limited mainly to type II pneumocytes.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Haagmans, B L" sort="Haagmans, B L" uniqKey="Haagmans B" first="B L" last="Haagmans">B L Haagmans</name><name sortKey="Kuiken, T" sort="Kuiken, T" uniqKey="Kuiken T" first="T" last="Kuiken">T. Kuiken</name><name sortKey="Leijten, L" sort="Leijten, L" uniqKey="Leijten L" first="L" last="Leijten">L. Leijten</name><name sortKey="Martina, B E E" sort="Martina, B E E" uniqKey="Martina B" first="B E E" last="Martina">B E E. Martina</name><name sortKey="Osterhaus, A D M E" sort="Osterhaus, A D M E" uniqKey="Osterhaus A" first="A D M E" last="Osterhaus">A D M E. Osterhaus</name><name sortKey="Van Riel, D" sort="Van Riel, D" uniqKey="Van Riel D" first="D" last="Van Riel">D. Van Riel</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Van Den Brand, J M A" sort="Van Den Brand, J M A" uniqKey="Van Den Brand J" first="J M A" last="Van Den Brand">J M A. Van Den Brand</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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